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KMID : 0613820170270121437
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Journal of Life Science
2017 Volume.27 No. 12 p.1437 ~ p.1444
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Anti-inflammatory Activities of an Ethanol Extract of Sargassum macrocarpum in Lipopolysaccharide (LPS)-stimulated RAW 264.7 Macrophages
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Cheon Ji-Min
Kim Hyang-Suk
Choi Eun-Ok
Kwon Da-Hye
Choi Young-Hyun
Kim Byung-Woo
Hwang Hye-Jin
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Abstract
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Sargassum macrocarpum is a widely distributed marine brown algae found in the North Pacific. The objective of this study was to evaluate the anti-inflammatory activity of an ethanol extract of S. macrocarpum (EESM). First, we investigated the anti-inflammatory activities of EESM in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. EESM treatment suppressed nitric oxide (NO) and prostaglandin E2 (PGE2) production and inhibited the expressions of the inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the mRNA and protein levels. In addition, the expression of pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α) and interleukin-1 beta (IL-1β), was decreased in a dose dependent manner. Investigation of the signaling pathways of nuclear factor kappa B (NF-ĸB), phosphoinositide-3-kinase (PI3K)/Akt, and mitogen-activated protein kinases (MAPKs) revealed suppression of NF-ĸB translocation from the cytosol to nucleus by EESM treatment. The phosphorylation of the Akt and ERK proteins was also inhibited by EESM treatment. EESM treatment also stimulated the expression of the heme oxygenase-1 (HO-1) enzyme and its upstream transcription factor, nuclear factor-E2-related factor 2 (Nrf2). These results suggest that EESM has anti-inflammatory activity and could have potential uses in the field of nutraceuticals.
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KEYWORD
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COX-2, iNOS, pro-inflammatory cytokines, RAW 264.7 macrophages, Sargassum macrocarpum
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